Prevalence of antibiotic resistance genes bla-CTX-M, bla-SHV, bla-TEM in enterobacteria strains isolated from perinatal center patients

Relevance A common mechanism of resistance is the synthesis enzymes that inactivate antibiotic (AB). Since its discovery at end 20th century, ESBLs have spread widely throughout world. Representatives this group complex protein compounds properties hydrolysis ß-lactam antibiotics and differ from each other in amino acid sequence. The genetic determinants determine their are bla-CTX-M, bla-SHV, bla-TEM genes. Aims. To assess prevalence genes antibiotic-resistant strains enterobacteria isolated samples patients perinatal center. Materials & Methods . We studied 135 ESBL-producing during microbiological monitoring January 01, 2020 to December 31, 2021. Biological material (feces (92), blood (1), urine (5), cervical canal discharge (33), last (4)) came center (43 women 92 newborns), examined both for clinical indications local monitoring. Species identification microorganisms was carried out by bacteriological method, detection using real-time PCR Results In 87 cases (64.4%), were successfully identified. Of 26 Klebsiella pneumonia, 24 (92.3%) able establish AB, 50% which represented bla-CTX-M. Out 66 Escherichia coli, 59 (89.33%) had a profile resistance, also dominated bla-CTX-M (67.80%). However, Enterobacter cloacae group, it possible presence only 8.10% cases. K. pneumoniae carrying three once sample woman aged 27 years. E. coli CTX-M gene 3 mother-child pairs cases: mothers – third trimester pregnancy, newborns feces first week life, can indicate transmission an AB-resistant microorganism either utero or through birth canal. Conclusions Thus, course study, identified 64.4% dominant leading occurrence AD among ESBL gene. number with established AB less (p<0.001). order study more detail identify mechanisms formation necessary expand observed